For example, you want to prepare 100 ml of 1 mM EDTA solution from 0.5M EDTA solution. Example: When the concentration of stock solution concentration is given in a concentration unit e.g. Take 10mL of the solution prepared in Step 1 and add water to 250m mark (250mL volumetric flask). So you need to mix 10 ml of 10X stock solution and 90 ml water to obtain 100 ml of working (diluted) solution.

Never store solutions in a volumetric flask. Transfer the prepared solution to a clean, dry storage bottle and label it.

Dissolve 1.054g of ferrous ammonium sulfate in 20mLof water,and add 1mLof sulfuric acid and 1mLof 30percent hydrogen peroxide.Mix,heat until effervescence ceases,and dilute with water to 50mL.To 3volumes of this solution contained in a volumetric flask add sulfuric acid,with cooling,to make 100volumes.Purify phenol by distillation,discarding the first 10%and the last 5%,collecting the distillate,with exclusion of moisture,in a dry,tared glass-stoppered flask of about twice the volume of the phenol.Solidify the phenol in an ice bath,breaking the top crust with a glass rod to ensure complete crystallization.Weigh the flask and its contents,add to the phenol 1.13times its weight of the ironsulfuric acid solution prepared as directed,insert the stopper in the flask,and allow to stand,without cooling but with occasional mixing,until the phenol is liquefied.Shake the mixture vigorously until mixed,allow to stand in the dark for 16to 24hours,and again weigh the flask and its contents.To the mixture add 23.5%of its weight of a solution of 100volumes of sulfuric acid in 110volumes of water,mix,transfer to dry glass-stoppered bottles,and store in the dark,protected from atmospheric moisture.Use within 6months.Dispense the reagent from a small-bore buret,arranged to exclude moisture,capable of delivering 1mLin 30seconds or less,and having no lubricant,other than reagent,on its stopcock.Wipe the buret tip with tissue before each addition. Take 10mL of 25 solution and dilute it to 1000mL mark 1000mL volumetric flask) with water. How to prepare 1000 ml of ammonium hydroxide To prepare 1000 mL of a 0.1 mol/L solution of Ammonium hydroxide we will need to dilute 6.73 mL of 58 NH4OH to a final volume with deionized (distilled) water.

How to prepare dilute ammonia solution